Calculate Polyacrylamide gel recipes for SDS-PAGE

This calculator is for the Hoefer™ SE 275 Mighty Small 4-Gel Caster (80-6151-06)

 

Enter Desired Gel Percent:
%
       
 
ml Total Volume
 
ml ddH2O
 
ml Acrylamide
 
ml 1.5M Tris pH 8.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
       
       
 
ml Stacking gel
 
ml ddH2O
 
ml % Acrylamide
 
ml 0.5M Tris pH 6.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
       
       

© 2007 Joseph T.E. Roland
www.Cytographica.com