Calculators Solutions Protocols Feedback Sitemap Calculators Convert between English and Metric Units Lazy Chemist's Calculator Henderson-Hasselbalch Calculator for Tris Buffers Desktop Centrifuge RCF / RPM Calculator NEW STUFF Polyethylenimine (PEI) Transfection Protocol LBE-5052 Autoinduction Media (for normal bacterial protein production) N-5052 Autoinduction Media (for N15-labeling) SDS-PAGE Recipe Calculators Calculate Recipes for SDS-PAGE gels by entering the desired percent polyacrylamide and total final volume Calculate Recipes for SDS-PAGE gels - gives both separating and stacking gel recipes Calculate Recipes for SDS-PAGE gel casting in a Hoefer™ SE 275 Mighty Small 4-Gel Caster Effectene Transfection Recipe Calculators Calculate Single Effectene Transfections for 12-well plates Calculate Effectene Co-Transfections for 12-well plates Calculate Single Effectene Transfections for 6-well plates or 35 mm dishes Calculate Single Effectene Transfections for 60 mm dishes Calculate Single Effectene Transfections for 10 cm dishes   Calculate Effectene Transfections for 12-well plates (up to 6 different DNAs) Calculate Effectene Transfections for 24-well plates (up to 6 different DNAs) Calculate Effectene Transfections for 6-well plates or 35 mm dishes (up to 6 different DNAs)   Solutions Common Buffers: PBS (Phosphate-buffered saline) TBS (Tris-buffered saline) Borate Buffer (for antibody storage) Autoinduction Media: LBE-5052 Autoinduction Media (for normal bacterial protein production) N-5052 Autoinduction Media (for N15-labeling) Stock Solutions: 1 M Tris-Cl pH 7.4 - 8.0 1.5 M Tris-Cl pH 6.8 5 M NaCl 1 M IPTG (isopropyl-1-thio-β-D-galactopyranoside) 20% SDS 20% Triton X-100 20% NP-40 20% Tween-20 10% CHAPS 10% sodium deoxycholate 1% bromophenol blue Immunocytochemistry Buffers: 4% PFA (Paraformaldehyde) 1% BSA Wash 5% Normal Serum Blocking Buffer 0.5% Fish Skin Gelatin Blocking Buffer SDS-PAGE / Western Blotting: RIPA (Radioimmunoprecipitation Assay) Buffer CHAPS Lysis Buffer Sample Buffer (for SDS-PAGE) Running Buffer (Towbin for SDS-PAGE) Transfer Buffer (for Western Blotting) TBST (Tris-buffered saline / Tween) BLOTTO (Bovine Lacto Transfer Technique Optimizer) Coomasie Stain Coomasie Destain DNA / RNA / Agarose gel buffers: Loading Dye (for DNA) TAE (Tris-acetate-EDTA) TBE (Tris-borate-EDTA) Yeast Two-Hybrid Buffers: YPDA (Yeast, Peptone, Dextrose, Adenine) 10X TE (Tris-EDTA) 10X LiAc (Lithium Acetate) 50% PEG (Polyethelene glycol) Z-Buffer (for β-galactosidase assays) X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) Antibiotics: Ampicillin (100 mg/ml stock) Carbenicillin (100 mg/ml stock) Chloramphenicol (34 mg/ml stock) Kanamycin (50 mg/ml stock) Protocols Common: Cell Culture Lysate Preparation and Immunoprecipitation for Dynal® Dynabeads®   Immunocytochemistry:   SDS-PAGE / Western Blotting: Coomasie Staining and Destaining for SDS-PAGE   Protein Production: Bacterial Protein Production - Test Induction   DNA / RNA:   Yeast Two-Hybrid:   Please leave feedback about these Lab Tools. Include corrections or additions that you feel may make this a more useful resource. Please make sure your email address is correct, or I can not reply to your requests. Your Name:   Your E-Mail Address:   Subject:     Comments:          Sorry, but this is REQUIRED now. It helps stop robotic spammers.
	© 2007 Joseph Roland