CHAPS Lysis Buffer

1% CHAPS Lysis Buffer - 100 ml

30 mM Tris-Cl pH 7.5, 150 mM NaCl, 1% CHAPS

Reagents needed:

3 ml	1 M Tris-Cl pH 7.5
3 ml	5 M NaCl
10 ml	10% CHAPS
84 ml	ddH₂O

Directions:

1) Add 3 ml of 1 M Tris-Cl to 84 ml of ddH₂O and mix.

2) Add 3 ml of 5 M NaCl and mix.

3) Add 10 ml of 10% CHAPS and mix.

5) Store at 4°C.

© 2007 Joseph T.E. Roland
www.Cytographica.com