Kanamycin - 10 mL (1,000 X Stock)

50 mg/ml kanamycin in water

Reagents needed:

0.5 g
kanamycin disulfate salt
10 ml
ddH2O

Directions:

1) Dissolve 0.5 g of kanamycin into 10 ml of ddH2O.

2) Filter through a 0.22 µm filter to sterilize.

3) Aliquot and store at -20°C.

4) Use at 1:1000 dilution in LB or LB-Agar.

© 2007 Joseph T.E. Roland
www.Cytographica.com