RIPA (RadioImmunoPrecipitation Assay) Buffer - ml  

50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS

Reagents needed:

5
ml 1 M Tris-Cl pH 7.4
3
ml 5 M NaCl
5
ml 20% NP-40
ml 10% sodium deoxycholate
0.5 ml 20% SDS
81.5 ml ddH2O

Directions:

1) Add Tris, NaCl, NP-40, sodium deoxycholate, and SDS to 81.5 ml of ddH2O and mix.

2) Store at 4°C.

 

 

© 2007 Joseph T.E. Roland
www.Cytographica.com