TBS (Tris-buffered saline) - 1L

25 mM Tris, 150 mM NaCl, 2 mM KCl, pH 7.4

  1X TBS 10X TBS
  Reagents needed: Reagents needed:
     
8 g
NaCl
80 g
NaCl
0.2 g
KCl
2 g
KCl
3 g
Tris base
30 g
Tris base
 
800 ml
ddH2O
800 ml
ddH2O

Directions:

1) Dissolve all dry reagents together in 800 ml of ddH2O.

2) Adjust the pH to 7.4 using HCl.

3) Add ddH2O to a final volume of 1 L.

4) Sterilize by autoclaving.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com