TE (Tris-EDTA) buffer (pH 7.4 to 8.0) - 1L


10 mM Tris, 1 mM EDTA - pH is determined by the pH of the Tris-Cl

  1X TE 10X TE
  Reagents needed: Reagents needed:
     
10 ml
100 ml
1 M Tris-Cl
2 ml
0.5 M EDTA pH 8.0
20 ml
0.5 M EDTA pH 8.0
 
988 ml
ddH2O
880 ml
ddH2O

Directions:

1) Mix Tris-Cl and EDTA with ddH2O.

2) Sterilize by autoclaving.

3) Store at 4°C or room temperature.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com