1 M Tris-Cl (pH 7.4 to pH 8.0) - 1 L
Reagents needed:
121.1 g |
Tris base |
700 ml |
ddH2O |
70 ml |
Concentrated HCl (37.2% - 12.1M) |
** NOTE: Many electronic pH Meters are unable to accurately determine the pH of concentrated Tris solutions. Be sure to use an appropriate probe. **
Directions:
1) Mix 121.1 g of Tris base with 700 ml of ddH2O by stirring.
2) Add HCl in the following volumes to reach the desired pH:
pH 7.4 = 70 ml of HCl; pH 7.6 = 60 ml of HCl; pH 8.0 = 42 ml of HCl
3) Fine adjust to the desired pH (7.4 - 8.0) with concentrated HCl.
4) Add ddH2O until final volume is 1 L.
5) Autoclave to sterilize.
Alternatively - use this Henderson-Hasselbalch calculator to determine the volume of HCl needed
© 2007 Joseph T.E. Roland
www.Cytographica.com