1 M Tris-Cl (pH 7.4 to pH 8.0) - 1 L


Reagents needed:

121.1 g
Tris base
700 ml
ddH2O
70 ml
Concentrated HCl (37.2% - 12.1M)

** NOTE: Many electronic pH Meters are unable to accurately determine the pH of concentrated Tris solutions. Be sure to use an appropriate probe. **

Directions:

1) Mix 121.1 g of Tris base with 700 ml of ddH2O by stirring.

2) Add HCl in the following volumes to reach the desired pH:

pH 7.4 = 70 ml of HCl; pH 7.6 = 60 ml of HCl; pH 8.0 = 42 ml of HCl

3) Fine adjust to the desired pH (7.4 - 8.0) with concentrated HCl.

4) Add ddH2O until final volume is 1 L.

5) Autoclave to sterilize.

Alternatively - use this Henderson-Hasselbalch calculator to determine the volume of HCl needed

 

© 2007 Joseph T.E. Roland
www.Cytographica.com