1000X Trace Metals Mix

1000X Trace Metals Mix - 100 ml

50mM FeCl₃, 20mM CaCl₂, 10mM MnCl₂, 10mM ZnSO₄, 2mM CoCl₂, 2mM CuCl₂, and 2mM NiCl₂

Directions:

1)
Add 0.5 ml of 10 N HCl to 49.5 ml of ddH2O to make 0.1 M HCl

2)
Dissolve 1.35 g of FeCl₃•6H₂O in the 50 ml of 0.1 M HCl  (F.W. of FeCl₃•6H₂O = 270.30)

3)
Add 36 ml of ddH₂O.

4)
Dissolve 294 mg of CaCl₂  (F.W. = 147.02)                or  Add 2 ml of 1 M CaCl₂

5)
Dissolve 198 mg of MnCl₂•4H₂O  (F.W. = 197.91)    or  Add 1 ml of 1 M MnCl₂

6)
Dissolve 288 mg of ZnSO₄•7H₂O  (F.W. = 287.56)   or  Add 1 ml of 1 M ZnSO₄

7)
Dissolve 48 mg of CoCl₂•6H₂O  (F.W. = 237.95)      or  Add 1 ml of 0.2 M CoCl₂

8)
Dissolve 34 mg of CuCl₂•6H₂O  (F.W. = 170.48)      or  Add 2 ml of 0.1 M CuCl₂

9)
Dissolve 48 mg of NiCl₂•6H₂O  (F.W. = 237.72)       or  Add 1 ml of 1 M NiCl₂

10)
Add ddH₂O up to 100 ml (∼14 ml if all dry reagents were used)

11)
Sterizlize the solution by passing it through a 0.22 µm filter

12)
Store at room temperature

Based on "Protein production by auto-induction in high-density shaking cultures", F. William Studier,
Protein Expression and Purfication, 41 (2005) 207-234

1)	Add 0.5 ml of 10 N HCl to 49.5 ml of ddH2O to make 0.1 M HCl
2)	Dissolve 1.35 g of FeCl₃•6H₂O in the 50 ml of 0.1 M HCl (F.W. of FeCl₃•6H₂O = 270.30)
3)	Add 36 ml of ddH₂O.
4)	Dissolve 294 mg of CaCl₂ (F.W. = 147.02) or Add 2 ml of 1 M CaCl₂
5)	Dissolve 198 mg of MnCl₂•4H₂O (F.W. = 197.91) or Add 1 ml of 1 M MnCl₂
6)	Dissolve 288 mg of ZnSO₄•7H₂O (F.W. = 287.56) or Add 1 ml of 1 M ZnSO₄
7)	Dissolve 48 mg of CoCl₂•6H₂O (F.W. = 237.95) or Add 1 ml of 0.2 M CoCl₂
8)	Dissolve 34 mg of CuCl₂•6H₂O (F.W. = 170.48) or Add 2 ml of 0.1 M CuCl₂
9)	Dissolve 48 mg of NiCl₂•6H₂O (F.W. = 237.72) or Add 1 ml of 1 M NiCl₂
10)	Add ddH₂O up to 100 ml (∼14 ml if all dry reagents were used)
11)	Sterizlize the solution by passing it through a 0.22 µm filter
12)	Store at room temperature