SDS-PAGE Running Buffer (Towbin)- 2 L

25 mM Tris, 192 mM glycine, 0.1% SDS

  1X Running Buffer 10X Running Buffer
  Reagents needed: Reagents needed:
     
28.8 g
glycine
288 g
glycine
6.04 g
Tris base
60.4 g
Tris base
2 g
SDS
20 g
SDS
 
1.8 L
ddH2O
1.8 L
ddH2O

** CAUTION ** SDS powder is hazerdous. Prepare solution in a ventilated fume hood.

Directions:

1) Dissolve Tris base and glycine together in 1.8 L of ddH2O.

2) Add SDS and mix.

3) Add ddH2O to a final volume of 2 L.

 

© 2007 Joseph T.E. Roland
www.Cytographica.com