Running Buffer ( for SDS-PAGE )

SDS-PAGE Running Buffer (Towbin)- 2 L

25 mM Tris, 192 mM glycine, 0.1% SDS

	1X Running Buffer	10X Running Buffer
	Reagents needed:	Reagents needed:

28.8 g	glycine	288 g	glycine
6.04 g	Tris base	60.4 g	Tris base
2 g	SDS	20 g	SDS
1.8 L	ddH₂O	1.8 L	ddH₂O

** CAUTION ** SDS powder is hazerdous. Prepare solution in a ventilated fume hood.

Directions:

1) Dissolve Tris base and glycine together in 1.8 L of ddH₂O.

2) Add SDS and mix.

3) Add ddH₂O to a final volume of 2 L.

© 2007 Joseph T.E. Roland
www.Cytographica.com