Coomassie Stain - 1 L

0.1% Coomassie R250, 10% acetic acid, 40% methanol

Reagents needed:

1 g
Coomassie R250
100 ml
glacial acetic acid
400 ml
methanol
500 ml
ddH2O

Directions:

1) Add 100 ml of glacial acetic acid to 500 ml of ddH2O.

2) Add 400 ml of methanol and mix.

3) Add 1g of Coomassie R250 dye and mix.

4) Filter to remove particulates (a coffee filter works great for this and is cheap)

5) Store at room temperature in a sealable container.

 

 

© 2007 Joseph T.E. Roland
www.Cytographica.com